1. Improvements to premium feature activation. 2. Bug fixes and performance enhancements.

‘Intrinsic Terminator’ is now called ‘Terminator’, reflecting support for both rho-independent and rho-dependent terminators.

Minor improvements to purchase flow and legacy account recognition.

A maintenance update with improvements to purchase handling and legacy user detection.

Improved purchase restore reliability.

Legacy BioCaL users now get premium features automatically, while new post-IAP users can purchase or restore premium seamlessly, with minor bug fixes and improvements.

Premium features unlock immediately. Purchase popup screen optimised for smaller displays. Manually restore purchases.

Added Premium upgrade, unlock advanced experimental tools.

1. Added lag time estimation to predict when cells will begin exponential growth after inoculation in the bacterial growth timer ODAlarm. 2. Introduced a growth curve generator in the growth tracking option for visualising culture progression. 3. Added a promoter classification feature to identify whether an input sequence is a promoter or non-promoter. 4. Added a ρ-independent terminator classification feature to identify whether an input sequence is a terminator or non-terminator.

Refined the popup alert message in the Tm calculator for pasted oligos exceeding 60 nucleotides.

Paste primers in Tm Calculator and get instant copy feedback.

1. New option to view the 3D structure of dinucleotide base stacking. 2. Reformatted the introduction page layout for improved display on iPad screens. 3. Redesigned button shapes for a more modern and tactile appearance.

Two options have been added to visualize the three-dimensional structure of deoxynucleic acids and their base pairs.

Reduced the default GC% from 90% to 79%, aligning with the naturally occurring GC content range. Most prokaryotic genomes fall within this range, with species like Escherichia coli K-12 having a GC content of around 50%, Thermus thermophilus HB27 reaching approximately 69%, and Micrococcus luteus NCTC 2665 up to 74%.

Average distance calculations are now performed directly as users vary motif sequence length, GC%, pA, and pC, with the 'Calculate' button removed.

Added a delete button to the motif sequence input section to facilitate seamless editing.

Users can specify the frequency of each nucleotide when calculating the average distance in "Average Distance II" under the Restriction Enzymes section.

Considering the overall GC content to evaluate the occurrence of each nucleotide when calculating the average distance.

Generalize the distance calculation algorithm to remove the restriction to a 6-nt sequence motif, enabling the calculation of distances for sequences of any length (up to 16 bp) and any combination.

Added a function to calculate the average distance between occurrences of a user-defined 6-nt sequence pattern, accounting for the degree of overlap within the pattern to provide a more accurate estimation of fragment length.

Display the results immediately after tapping the Calculate button.

Added an option to estimate the remaining antibiotic-resistant population as a function of generation number by inactivating the antibiotic resistance gene at each generation using a base editor.

Resolved an issue where the popup menu, which functioned correctly in Xcode 15.x, was not working as expected in Xcode 16.2. This fix has been implemented in version 2.32.49.

The population fraction with the largest number gets the maximum width, and the others are scaled accordingly to effectively capture the distribution in the mutation fixation option.

Fixed a bug that ensures the identical matrix is correctly calculated at generation zero in the mutation fixation function.