Changes introduced in NEBtools v2.6.7: The restriction enzyme data for Enzyme Finder and Double Digest Finder were updated. NEB Customer Service was revised to Customer Support.

Version 2.6.5 corrects an error in processing the on-board data used by the app which resulted in an incorrect incubation temperature for enzyme SmaI.

Version 2.6.4 of NEBtools updates th on-board restriction enzyme data set.

Version 2.6.1 adds support for Q5 Blood Direct 2x Master Mix in Tm Calculator. Revisions were made to the effective monovalent cation concentration associated with some of the buffers used in Tm Calculator. Minor updates were also made to fix bugs.

Version 2.5.0 updates the restriction enzyme data used by Enzyme Finder and Double Digest. Minor updates were also made to fix reported bugs.

Version 2.4.0 of NEB Tools updates the on-board restriction enzyme data used by Double Digest and Enzyme Finder. The update also includes the new naming scheme for NEBuffers reflecting the new BSA-free formulations. Enzyme supplement information was also updated.

This version updates the restriction enzyme data used by Double Digest Finder and Enzyme Finder. It also includes minor updates to protocols and minor correction to the biocalculator modules. This release also addresses data issue that might cause crashes in version 2.3.0.

This version updates the restriction enzyme data used by Double Digest Finder and Enzyme Finder. It also includes minor updates to protocols and minor correction to the biocalculator modules.

This version of NEB Tools updates the restriction enzyme data and digest protocols used by Enzyme Finder and Double Digest. Single enzyme digest protocols are easier to obtain from within Double Digest Finder. It is recommended for all existing users.

NEBtools v2.1.2 fixes several incompatibilty issues with iOS 13 affecting the Double Digest Finder and NEBioCalculator modules.

This version of NEBtools corrects an issue in the NEBioCalculator module that could lead to apparently incorrect results. The issue resolves automatic changes to the units on certain calculator inputs after the first input value is entered. These automatic changes may be easily overlooked. The changes have been disabled.

Release v2.1.0 of NEBtools includes updates to the enzyme data, minor feature enhancements to Tm Calculator and NEBioCalculator modules, as well as bug fixes for identified problems that could crash the application under certain conditions.

NEBtools has been updated to work on both iPhone and iPad running the latest versions of iOS and includes many design enhancements and new functionality. Redesigned Enzyme Finder and Double Digest Finder allow easier enzyme selection. Double Digest Finder includes detailed digestion protocols. NEBtools v2.0.0 also includes several popular calculators from the NEBioCalculator web app.

Version 1.7.2 of NEB Tools correctly handles number formats in regions using commas in place of decimal points. This allows proper setting of amplicon size in the PCR cycler settings view.

Corrected US tech support phone number. Upgrades mentioned in v 1.7.0 were maintained. NEB Tools now includes the NEB Tm Calculator for determining optimal annealing temperatures and suggested cycler settings for PCR reactions using NEB polymerases and buffers. The main screen now features easy ways to contact NEB's technical support service. Release 1.7.0 also includes minor updates to the restriction enzyme data used in the Double Digest Finder.

NEB Tools now includes the NEB Tm Calculator for determining optimal annealing temperatures and suggested cycler settings for PCR reactions using NEB polymerases and buffers. The main screen now features easy ways to contact NEB's technical support service. Release 1.7.0 also includes minor updates to the restriction enzyme data used in the Double Digest Finder.

This version adds conversions between the old and new NEBuffer systems in Double Digest Finder as an aid to users who need to continue using their stocks of old NEBuffers.

Updated Enzyme Finder and Double Digest Finder to work with NEB's new and improved restriction enzyme buffers, NEBuffer 1.1, 2.1, 3.1 and CutSmart.

Updated enzyme database. Resolved potential error when searching for asymmetric recognition sequences. Updated to work with iPhone 5 and iOS 6.

Contains updated restriction enzyme database incorporating new enzymes and buffer changes. New High Fidelity enzymes. RE-Mix Master Mix enzymes. Restored missing R/Y keys in sequence editor. Minor corrections to Double Digest Calculator algorithm.

Contains updated restriction enzyme database. Minor bug fixes. Supports iOS 4.3 Last version to support iOS 3.1.3.

Corrected display of methylated bases in the recognition sequences of EpiMark(TM)-validated enzymes. Tested for compatibility with iOS 4.2.

Version 1.3.0 is a maintenance release that updates the database and includes a new search category for EpiMark(TM) validated restriction enzymes that are useful in epigenetic analysis.

Double digestion data has been updated. Compatibility with iOS4 tested.

·     View NEB product web page without leaving the app ·     5 newly released HF restriction enzymes added ·     Incorporates updated buffer activity data ·     Ability to override Double Digest Finder warnings & view requested data